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KMID : 0848120060310010021
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International Journal of Oral Biology
2006 Volume.31 No. 1 p.21 ~ p.26
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Cyclooxygenase-2 Induction in Porphyromonas gingivalis-Infected THP-1 Monocytic cells
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Choi Eun-Kyoung
Oh Byung-Ho
Kang In-Chol
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Abstract
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Periodontopathogens including Porphyromonas gingivalis interact with host periodontal cells and the excessive subsequent host responses contribute a major part to the development of periodontal diseases. Cyclooxygenase(COX)-2-synthesized PGE₂has detrimental activities in terms of periodontal pathogenesis. The present study investigated induction of COX-2 expression by P. gingivalis in human monocytic THP-1 cells. Live P. gingivalis increased expression of COX-2, but not that of COX-1, which was demonstrated at both mRNA and protein levels. Elevated levels of PGE₂were released from P. gingivalis-infected THP-1 cells. Pharma-cological inhibition of p38 mitogen-activated protein kinase(MAPK) and extracellular signal-regulated kinase(ERK) substantially attenuated P. gingivalis-induced COX-2 mRNA expression. Indeed, activation of p38 MAPK and ERK was observed in P. gingivalis-infected THP-1 cells. Also, P. gingivalis induced activation of nuclear factor-κB (NF-κB) which is an important transcription factor for COX-2. These results suggest that COX-2 expression is up regulated in P. gingivalis-infected monocytic cells, at least in part, via p38 MAPK, ERK, and NF-κB.
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KEYWORD
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Porphyromonas gingivalis, cyclooxygenase-2, signaling, THP-1
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